The cellulase complex of Neurospora crassa: cbh-1 cloning, sequencing and homologies

Gene. 1995 Aug 8;161(1):137-8. doi: 10.1016/0378-1119(95)00288-h.

Abstract

We describe the isolation, cloning and sequencing of the cellobiohydrolase 1 (EC 3.2.1.91)-encoding gene (cbh-1) of Neurospora crassa. The nucleotide and amino-acid sequences have high homology with the cbh-1 of Trichoderma reesei, Humicola grisea and Phanerochaete chrysosporium, with clear signal, catalytic, hinge and substrate-binding domains in that order.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cellulose 1,4-beta-Cellobiosidase
  • Cloning, Molecular
  • DNA Primers
  • Genes, Fungal
  • Glycoside Hydrolases / chemistry
  • Glycoside Hydrolases / genetics*
  • Molecular Sequence Data
  • Neurospora crassa / enzymology*
  • Neurospora crassa / genetics
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid

Substances

  • DNA Primers
  • Glycoside Hydrolases
  • Cellulose 1,4-beta-Cellobiosidase

Associated data

  • GENBANK/S42093