Induction of micronuclei in V79 Chinese hamster cells by hydroquinone and econazole nitrate

Mutat Res. 1993 May;287(1):87-91. doi: 10.1016/0027-5107(93)90147-8.

Abstract

Two suspect aneugens (hydroquinone and econazole nitrate) were examined for their ability to induce micronuclei in a number of V79 Chinese hamster cell lines which express rat cytochrome P-450 cDNAs. Hydroquinone elevated micronucleated cell frequencies in a dose-dependent manner in cell lines V79, XEM2 (expresses CYP1A1) and SD1 (expresses CYP2B1). Econazole nitrate was an effective inducer of micronuclei over a narrow dose range in cell lines V79, XEM2 and XEMd-MZ (expresses CYP1A2). The different cell lines showed similar responses to the test agents, indicating that hydroquinone is not a substrate for biotransformation by rat CYP1A1 or CYP2B1, nor is econazole nitrate biotransformed by rat CYP1A1 or CYP1A2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cricetinae
  • Cricetulus
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism
  • Dose-Response Relationship, Drug
  • Econazole / toxicity*
  • Hydroquinones / toxicity*
  • Micronucleus Tests / methods*
  • Mutagens / toxicity*
  • Rats
  • Transfection

Substances

  • Hydroquinones
  • Mutagens
  • Econazole
  • Cytochrome P-450 Enzyme System
  • hydroquinone