Overexpression of c-kit in a leukemic cell population carrying a trisomy 4 and its relationship with the proliferative capacity

Leuk Lymphoma. 1993 Apr;9(6):495-501. doi: 10.3109/10428199309145756.

Abstract

The expression of c-kit and its ligand, the stem cell factor (SCF), was studied in five cases of acute myeloid leukemia. One of these had a trisomy of chromosome 4, where the c-kit oncogene is located. In this case, the c-kit oncogene was overexpressed, but matched by a low expression of its ligand, SCF. The molecular evaluation of the growth rate by c-myc and the histone H3 expression indicated that the growth fraction of this cell population was very low. In one of the other leukemic cell populations studied, characterized by a low expression of c-kit and an elevated expression of the SCF, the growth fraction was also very low. Our results suggest that at least for some receptor oncogenes, the simple overexpression cannot be taken as an indication that the oncogene is involved in the deregulation of cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Division
  • Chromosome Mapping
  • Chromosomes, Human, Pair 4*
  • Gene Expression Regulation, Leukemic*
  • Genes, myc
  • Hematopoietic Cell Growth Factors / genetics
  • Histones / genetics
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / pathology
  • Molecular Sequence Data
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-kit
  • Proto-Oncogenes*
  • Stem Cell Factor
  • Trisomy*
  • beta 2-Microglobulin / genetics

Substances

  • Hematopoietic Cell Growth Factors
  • Histones
  • Proto-Oncogene Proteins
  • Stem Cell Factor
  • beta 2-Microglobulin
  • Proto-Oncogene Proteins c-kit