Abstract
Lim1 is a homeobox gene expressed in the organizer region of mouse embryos. To investigate the role of Lim1 during embryogenesis, a targeted deletion of the Lim1 gene was generated in embryonic stem cells. Embryos homozygous for the null allele lacked anterior head structures but the remaining body axis developed normally. A partial secondary axis developed anteriorly in some mutant embryos. Lim1 is thus an essential regulator of the vertebrate head organizer.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Base Sequence
-
Biomarkers
-
Brain / cytology
-
Brain / embryology
-
DNA Primers
-
Embryonic Induction / physiology*
-
Embryonic and Fetal Development / genetics
-
Embryonic and Fetal Development / physiology*
-
Gene Deletion
-
Gene Expression
-
Gene Targeting
-
Genes, Homeobox
-
Head / embryology*
-
Homeodomain Proteins / genetics
-
Homeodomain Proteins / physiology*
-
LIM-Homeodomain Proteins
-
Mesoderm
-
Mice
-
Mice, Inbred C57BL
-
Molecular Sequence Data
-
Phenotype
-
Stem Cells
-
Transcription Factors
Substances
-
Biomarkers
-
DNA Primers
-
Homeodomain Proteins
-
LIM-Homeodomain Proteins
-
Lhx1 protein, mouse
-
Transcription Factors