The dynamic properties of membrane protein are closely correlated to the membrane functions in erythrocytes. The anion transport function of band 3 protein, the constituents and the translational diffusion of membrane protein was detected in 25% TBSA burn rats. The changes in membrane protein SH were investigated by using the spin label of 3-maleimide-proxyl. The results showed that, in delayed fluid resuscitation group after burn shock, membrane crosslinking protein appeared in erythrocytes, the function of band 3 protein was damaged, the recovery time after fluorescence photobleaching of membrane protein was obviously prolonged, and the ratio of peak heights of strong to weak immobilization was increased, especially in the six-hour delayed resuscitation group, suggesting that the ischemia-reperfusion injury was more serious during the early shock stage. The authors believe that the results may represent the mechanism of damage biological membrane after burns. The alterations of membrane properties caused by free radicals play an important role in erythrocyte injury.