Detection of PML-RAR alpha fusion transcript in Ph positive leukemia with acute promyelocytic phenotype lacking the t(15;17) cytogenetic abnormality

G Emilia; R Marasca; G Longo; M G Ferrari; M Notohamiprodjo; P Temperani; S Sacchi; G Torelli

doi:10.1016/0165-4608(94)00147-4

Detection of PML-RAR alpha fusion transcript in Ph positive leukemia with acute promyelocytic phenotype lacking the t(15;17) cytogenetic abnormality

Cancer Genet Cytogenet. 1995 Apr;80(2):95-9. doi: 10.1016/0165-4608(94)00147-4.

Authors

G Emilia¹, R Marasca, G Longo, M G Ferrari, M Notohamiprodjo, P Temperani, S Sacchi, G Torelli

Affiliation

¹ Center for Experimental Hematology, University of Modena, Italy.

PMID: 7736444
DOI: 10.1016/0165-4608(94)00147-4

Abstract

A 39-year-old woman was diagnosed with acute promyelocytic leukemia (APL) with disseminated intravascular coagulation syndrome. The hematologic examination showed a morphologic, cytochemical, and immunophenotypic picture typical of an APL, with a marked leukocytosis and a mixed population of hypergranular and microgranular promyelocytes. The cytogenetic analysis showed a 46,XX,t(9;22) karyotype, without any alterations of chromosomes 15 and 17. The t(15;17) translocation was not evident in FISH experiments, while a molecular analysis revealed the presence of a PML-RAR alpha chimera.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Base Sequence
Chromosomes, Human, Pair 15*
Chromosomes, Human, Pair 17*
Chromosomes, Human, Pair 22
Chromosomes, Human, Pair 9
DNA Primers
Female
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
Leukemia, Promyelocytic, Acute / genetics*
Molecular Sequence Data
Phenotype
RNA, Messenger / genetics
Receptors, Retinoic Acid / genetics*
Recombinant Fusion Proteins / genetics
Translocation, Genetic*

Substances

DNA Primers
RNA, Messenger
Receptors, Retinoic Acid
Recombinant Fusion Proteins