[The multidrug resistance phenotype: some morphological and biological characteristics except efflux pump]

Bull Cancer. 1994 May;81(5):392-9.
[Article in French]

Abstract

Recent data from the literature together with personal results strongly suggest that multidrug resistance phenotype is overwhelming the sole expression of P170 glycoprotein efflux pump. Morphological alterations have been put in evidence in MDR cells after transmission and scanning electron microscopy. They include presence of osmiophilic vesicles and modifications of nuclear and nucleolar chromatin. Biological characteristics include the hypersecretory pattern of lysosomal enzymes from MDR cells. Such a fact could be more or less related to the increased occurrence of mdr1 RNA in metastasis, especially in breast cancers, compared to primary tumors. If the P170-mediated efflux is one of the key mechanism of MDR, a decreased influx of anticancer drugs cannot be excluded. Liposomes, for instance made of cardiolipin, are thus able to increase the intracellular drug uptake of vinblastine without any action upon efflux mechanism.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / drug effects*
  • Antineoplastic Agents / pharmacology*
  • Drug Carriers
  • Drug Resistance, Multiple* / genetics
  • Drug Resistance, Multiple* / physiology
  • Gene Expression*
  • Humans
  • Liposomes
  • Lysosomes / enzymology
  • Models, Biological
  • Neoplasm Metastasis
  • Phenotype
  • Tumor Cells, Cultured / drug effects
  • Vinblastine / pharmacology

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antineoplastic Agents
  • Drug Carriers
  • Liposomes
  • Vinblastine