Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin

J Clin Microbiol. 1995 Mar;33(3):629-30. doi: 10.1128/jcm.33.3.629-630.1995.

Abstract

Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anion Exchange Resins
  • Base Sequence
  • DNA-Directed DNA Polymerase
  • Humans
  • Molecular Sequence Data
  • Mycobacterium bovis / genetics
  • Mycobacterium bovis / isolation & purification
  • Mycobacterium tuberculosis / genetics
  • Mycobacterium tuberculosis / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Sputum / microbiology*

Substances

  • Anion Exchange Resins
  • DNA-Directed DNA Polymerase