Light induction of the clock-controlled gene ccg-1 is not transduced through the circadian clock in Neurospora crassa

Mol Gen Genet. 1995 Apr 20;247(2):157-63. doi: 10.1007/BF00705645.

Abstract

Ambient light and the circadian clock have been shown to be capable of acting either independently or in an interrelated fashion to regulate the expression of conidiation in the ascomycete fungus Neurospora crassa. Recently several molecular correlates of the circadian clock have been identified in the form of the morning-specific clock-controlled genes ccg-1 and ccg-2. In this paper we report studies on the regulation of ccg-1, an abundantly expressed gene displaying complex regulation. Consistent with an emerging consensus for clock-controlled genes and conidiation genes in Neurospora, we report that ccg-1 expression is induced by light, and show that this induction is independent of the direct effects of light on the circadian clock. Although circadian regulation of the gene is lost in strains lacking a functional clock, expression of ccg-1 is still not constitutive, but rather fluctuates in concert with changes in developmental potential seen in such strains. Light induction of ccg-1 requires the products of the Neurospora wc-1 and wc-2 genes, but surprisingly the requirement for wc-2 is suppressed in conditional mutants of cot-1, a gene that encodes, a cAMP-dependent protein kinase. These data provide insight into a complex regulatory web, involving at least circadian clock control, light control, metabolic control, and very probably developmental regulation, that governs the expression of ccg-1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Northern
  • Circadian Rhythm / genetics*
  • DNA, Complementary
  • Gene Expression
  • Genes, Fungal
  • Light*
  • Mutation
  • Neurospora crassa / genetics*
  • Neurospora crassa / physiology
  • RNA, Fungal / genetics
  • RNA, Fungal / isolation & purification

Substances

  • DNA, Complementary
  • RNA, Fungal