Abstract
The BCL-6 gene is known to be located on chromosome 3q27, at the breakpoint of the 3q27-associated translocations that occur frequently in human non-Hodgkin's lymphomas (NHLs). To identify the BCL-6 protein, two antibodies that recognized distinct domains of this protein were raised in rabbits. Immunoprecipitation and immunoblotting of lysates of BCL-6-expressing cells using both antibodies showed a broad 92- to 98-kD band. Dephosphorylation of BCL-6 protein reduced the size of this band to 87 kD, suggesting that BCL-6 may be expressed in a phosphorylated form. Immunostaining with both antibodies showed that BCL-6 protein was localized in the nuclei of most of the germinal center B cells and a small number of marginal zone B cells. Furthermore, BCL-6 protein was expressed in follicular, Burkitt's, and diffuse large B-cell lymphomas. These results suggest that the BCL-6 protein, expressed in B cells of the germinal centers which are important in the maturation of immune responses, may play some physiological role(s) in the germinal center B cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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B-Lymphocyte Subsets / metabolism*
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Base Sequence
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Bone Neoplasms / genetics
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Bone Neoplasms / metabolism
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Bone Neoplasms / pathology
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Burkitt Lymphoma / genetics
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Burkitt Lymphoma / metabolism
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Burkitt Lymphoma / pathology
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Cell Nucleus / metabolism
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DNA-Binding Proteins / biosynthesis*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / immunology
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Gene Expression Regulation, Leukemic
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Gene Expression Regulation, Neoplastic*
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Leukemia / genetics
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Leukemia / metabolism
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Leukemia-Lymphoma, Adult T-Cell / genetics
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Leukemia-Lymphoma, Adult T-Cell / metabolism
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Leukemia-Lymphoma, Adult T-Cell / pathology
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Lymphoma, B-Cell / genetics
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Lymphoma, B-Cell / metabolism
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Lymphoma, Large B-Cell, Diffuse / genetics
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Lymphoma, Large B-Cell, Diffuse / metabolism
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Lymphoma, Non-Hodgkin / genetics
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Lymphoma, Non-Hodgkin / metabolism*
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Mice
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Mice, SCID
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Molecular Sequence Data
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Neoplasm Proteins / biosynthesis*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / immunology
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Osteosarcoma / genetics
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Osteosarcoma / metabolism
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Osteosarcoma / pathology
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Palatine Tonsil / cytology*
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Phosphorylation
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Protein Processing, Post-Translational
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / immunology
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Proto-Oncogene Proteins c-bcl-6
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Rabbits
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Transcription Factors / biosynthesis*
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Transcription Factors / genetics
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Transcription Factors / immunology
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Tumor Cells, Cultured
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Tunicamycin / pharmacology
Substances
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Antibodies, Monoclonal
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DNA-Binding Proteins
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Neoplasm Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-6
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Transcription Factors
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Tunicamycin