Escherichia coli NusG protein stimulates transcription elongation rates in vivo and in vitro

J Bacteriol. 1995 Mar;177(5):1388-92. doi: 10.1128/jb.177.5.1388-1392.1995.

Abstract

The rate of transcription elongation in Escherichia coli was reduced when cells were depleted of NusG. In a purified in vitro system, NusG accelerated the transcription elongation rate. The stimulation of the rate of transcription elongation by NusG appears to result from the suppression of specific transcription pause sites.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • Base Sequence
  • Calmodulin-Binding Proteins / genetics
  • Drosophila Proteins*
  • Escherichia coli / genetics*
  • Escherichia coli Proteins*
  • Kinetics
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Peptide Elongation Factors / genetics*
  • RNA, Messenger / biosynthesis*
  • Suppression, Genetic
  • Transcription Factors / genetics*
  • Transcription, Genetic*
  • Transient Receptor Potential Channels

Substances

  • Bacterial Proteins
  • Calmodulin-Binding Proteins
  • Drosophila Proteins
  • Escherichia coli Proteins
  • Membrane Proteins
  • NusG protein, E coli
  • Peptide Elongation Factors
  • RNA, Messenger
  • Transcription Factors
  • Transient Receptor Potential Channels
  • trpl protein, Drosophila