Synthesis and primer properties of oligonucleotides containing 3'-deoxypsicothymidine units, labeled with fluorescein at the 1'-position

Bioconjug Chem. 1994 Nov-Dec;5(6):501-3. doi: 10.1021/bc00030a003.

Abstract

Several analogues of the standard M13 sequencing primer that contain up to five 3'-deoxypsicothymidines, or one or two such units labeled with fluorescein at the 1'-position, have been prepared. All these oligonucleotides have been shown to prime the DNA-polymerase-catalyzed synthesis of DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoradiography
  • Base Sequence
  • Chromatography, High Pressure Liquid
  • DNA / biosynthesis
  • DNA Primers / chemical synthesis*
  • DNA-Directed DNA Polymerase / metabolism
  • Fluorescein
  • Fluoresceins*
  • Fluorescent Dyes*
  • Molecular Sequence Data
  • Spectrophotometry, Ultraviolet
  • Thymidine / analogs & derivatives*

Substances

  • 3'-deoxypsicothymidine
  • DNA Primers
  • Fluoresceins
  • Fluorescent Dyes
  • DNA
  • DNA-Directed DNA Polymerase
  • Fluorescein
  • Thymidine