The circadian and circannual secretory patterns of growth hormone (GH) and prolactin (PRL) in Fresian heifers were analysed to identify the significant pulses, the shape and the periodic components of the pulsatile signal that may be relevant to intercellular communication. The possible influence of temperature and photoperiod over these hormonal secretions has also been investigated. In the bovine GH (bGH) secretory patterns the basal levels and number, magnitude and amplitude of significant secretory peaks seem to be independent and not affected by seasonal changes. All the bGH circadian patterns show two periodic components with distinct frequencies. The bovine PRL (bPRL) circadian secretion shows basal levels, number and magnitude of the secretory peaks greater in summer than in winter; the periodic components are only one in winter, but two in summer. There is a significant increase of plasma bPRL in the May-July period and a correlation of the circannual hormone profile with both temperature and daylength.