[Function of the calcium-binding protein p26 (recoverin) in bovine retinal rods]

Biokhimiia. 1994 Apr;59(4):582-8.
[Article in Russian]

Abstract

The bovine retina rod cell protein with an apparent M(r) 26 kDa (p26 [1] or recoverin [2]) was suggested to be a calcium-sensitive regulator of photoreceptor guanylate cyclase. The data obtained show that highly purified p26 is not capable of restoring guanylate cyclase in washed ROS membranes up to the level of a ROS suspension. At the same time we found that a Ca(2+)-sensitive complex of p26 and a protein with apparent M(r) 67 kDa, presumably rhodopsin kinase, is present in the ROS extract. We suggest that rhodopsin kinase can be a functional target for p26 in retina rod cells.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Calcium-Binding Proteins / metabolism*
  • Cattle
  • Chromatography, Gel
  • Eye Proteins*
  • G-Protein-Coupled Receptor Kinase 1
  • Guanylate Cyclase / metabolism
  • Hippocalcin
  • Lipoproteins*
  • Nerve Tissue Proteins*
  • Protein Kinases / metabolism
  • Recoverin
  • Retinal Rod Photoreceptor Cells / enzymology
  • Retinal Rod Photoreceptor Cells / metabolism*
  • Substrate Specificity

Substances

  • Calcium-Binding Proteins
  • Eye Proteins
  • Lipoproteins
  • Nerve Tissue Proteins
  • Recoverin
  • Hippocalcin
  • Protein Kinases
  • G-Protein-Coupled Receptor Kinase 1
  • Guanylate Cyclase