Abstract
Objective:
To study the Ig genes that encode IgG rheumatoid factor (IgG-RF) from rheumatoid synovial fluid.
Methods:
We used rheumatoid synovial fluid B cells to generate IgG-RF-secreting hybridomas. We then characterized their binding properties and determined their nucleotide sequences.
Results:
Two monospecific IgG-RFs were obtained. Sequence analysis of the RFs revealed a new V lambda gene family (designated V lambda 9) and extensive somatic diversification, including a duplication-insertion of 18 nucleotides (6 amino acid residues) into a hypervariable region.
Conclusion:
The data provide further support for an antigen-driven response in the sustained production of potentially pathogenic IgG-RFs in rheumatoid synovium.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Arthritis, Rheumatoid / immunology*
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B-Lymphocytes / metabolism
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Base Sequence
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DNA / analysis
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Humans
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Hybridomas / metabolism
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Light Chains / genetics
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Immunoglobulin M / immunology*
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Immunoglobulin Variable Region / genetics
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Molecular Sequence Data
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Polymerase Chain Reaction
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Rheumatoid Factor / analysis*
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Rheumatoid Factor / metabolism
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Synovial Fluid / immunology*
Substances
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Immunoglobulin M
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Immunoglobulin Variable Region
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DNA
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Rheumatoid Factor
Associated data
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GENBANK/L06914
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GENBANK/L06915
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GENBANK/L06916
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GENBANK/L06917
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GENBANK/L06918
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GENBANK/L06919
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GENBANK/L22586
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GENBANK/L22587
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GENBANK/L22588
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GENBANK/L22589