Persistence of O6-guanine DNA adducts in styrene-exposed lamination workers determined by 32P-postlabelling

Carcinogenesis. 1994 Sep;15(9):1949-53. doi: 10.1093/carcin/15.9.1949.

Abstract

A modified 32P-postlabelling method was used for the detection of styrene-specific DNA adducts in lamination workers. The persistence of O6-styrene DNA adducts was studied in DNA from lymphocytes and granulocytes of an exposed and a control group. We compared O6-adduct levels obtained from a sampling prior to vacation, after 2 weeks of vacation and after an additional 1 month of work. In granulocytes, there was no significant difference in adduct levels between the control and the exposed groups in any individual samplings. In lymphocytes of laminators the detected adduct levels were significantly higher (5.4 adducts/10(8) nucleotides) than those in the controls (1.0 adduct/10(8) nucleotides). The 2 week interruption of exposure did not influence the total O6-adduct level (4.9 adducts/10(8) nucleotides in the first sampling versus 5.1 adducts/10(8) nucleotides in the second), indicating very slow removal of the specific O6-styrene adducts from DNA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chromatography, Thin Layer
  • DNA Adducts / analysis
  • DNA Adducts / blood*
  • DNA Damage
  • Female
  • Granulocytes / chemistry
  • Granulocytes / metabolism
  • Guanine / analysis
  • Guanine / blood*
  • Humans
  • Lymphocytes / chemistry
  • Lymphocytes / metabolism
  • Male
  • Mandelic Acids / urine
  • Occupational Exposure*
  • Styrenes / adverse effects*
  • Styrenes / analysis
  • Styrenes / blood*

Substances

  • DNA Adducts
  • Mandelic Acids
  • Styrenes
  • Guanine
  • mandelic acid