p53 mutant His175 identified in a newly established fallopian tube carcinoma cell line secreting interleukin 6

FEBS Lett. 1994 Oct 10;353(1):29-32. doi: 10.1016/0014-5793(94)00953-8.

Abstract

Fallopian tube carcinoma is a lethal gynecologic malignancy. Etiologic factors are unknown. No experimental data on molecular alterations exist so far. For an in vitro model, we established the permanent human tubal carcinoma cell line FT-MZ-1. The median doubling time was 14 days with 24.2% in S phase. A point missense mutation of the p53 tumor suppressor gene resulting in the His175 mutant was identified. Aberrant p53 protein accumulated in nucleus and cytoplasm. FT-MZ-1 substantially secreted interleukin 6 (Il-6) coinciding with the inactivation of p53 as a transrepressor on the Il-6 gene promoter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Carcinoma / genetics*
  • Carcinoma / metabolism
  • DNA Primers
  • Fallopian Tube Neoplasms / genetics*
  • Fallopian Tube Neoplasms / metabolism
  • Female
  • Genes, p53*
  • Histidine / genetics
  • Humans
  • Interleukin-6 / metabolism*
  • Middle Aged
  • Molecular Sequence Data
  • Point Mutation*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Tumor Cells, Cultured

Substances

  • DNA Primers
  • Interleukin-6
  • Histidine