Evaluation of the effect of Sm28GST-derived peptides in murine hepatosplenic schistosomiasis: interest of the lipopeptidic form of the C-terminal peptide

Mol Immunol. 1994 Nov;31(16):1247-56. doi: 10.1016/0161-5890(94)90075-2.

Abstract

Among the synthetic peptides derived from the 28-kDa Schistosoma mansoni glutathione S-transferase (Sm28GST), immunization with the C-terminal peptide comprising amino acid residues 190-211 induced a reduction in splenomegaly, in the number of hepatic eggs and in hepatic fibrosis in mice infected by Schistosoma mansoni. The absence of antibodies specific for the Sm28GST or for the 190-211 peptide observed in our conditions of immunization with this peptide argued in favour of the involvement of cellular-dependent mechanisms in the reduction in hepatic pathology. This was confirmed by the passive transfer of 190-211 peptide-specific T-cell enriched spleen cells which reproduced the protective effect conferred by immunization with the 190-211 peptide. These 190-211 peptide-specific cells produced little IL4 and high levels of IFN-gamma, a potent inhibitor of collagen synthesis. Furthermore, the use of a lipopeptidic form of the 190-211 peptide significantly improved the reduction in hepatic pathology obtained with the uncoupled peptide and induced a durable protective response. These results provide encouraging information for the possible use of synthetic peptides in the immunoprophylaxis of Schistosomiasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Helminth / biosynthesis
  • Female
  • Glutathione Transferase / immunology*
  • Liver Diseases, Parasitic / prevention & control
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Parasite Egg Count
  • Peptide Fragments / immunology
  • Schistosoma mansoni / enzymology*
  • Schistosoma mansoni / immunology*
  • Schistosomiasis mansoni / prevention & control*
  • Splenic Diseases / parasitology
  • Splenic Diseases / prevention & control
  • T-Lymphocytes / transplantation
  • Vaccines, Synthetic* / immunology

Substances

  • Antibodies, Helminth
  • Peptide Fragments
  • Vaccines, Synthetic
  • Glutathione Transferase