LPS-inducible nuclear factor in human monocytes that binds the negative regulatory element of the HIV LTR

J Leukoc Biol. 1994 Jul;56(1):21-6. doi: 10.1002/jlb.56.1.21.

Abstract

We studied the constitutive and lipopolysaccharide (LPS)-induced expression of nuclear protein binding to the negative regulatory element (NRE) of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in fresh human monocytes. We demonstrated the existence of a constitutive factor binding to the NRE 73-bp HpaII/HpaII fragment (-216 to -143) whose expression is up-regulated by LPS treatment. Competition experiments with overlapping oligonucleotides covering the HpaII/HpaII fragment and with mutated oligonucleotides mapped the binding within the TTTCATCAC region (-171 to -163). This binding pattern is unique to human monocytes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cells, Cultured
  • DNA, Viral / analysis
  • DNA, Viral / genetics
  • DNA, Viral / metabolism
  • HIV / genetics
  • HIV Long Terminal Repeat / genetics*
  • HIV Long Terminal Repeat / physiology
  • Humans
  • Lipopolysaccharides / pharmacology*
  • Molecular Sequence Data
  • Monocytes / cytology
  • Monocytes / metabolism*
  • Nuclear Proteins / metabolism*
  • Protein Binding
  • Up-Regulation / drug effects
  • Up-Regulation / physiology

Substances

  • DNA, Viral
  • Lipopolysaccharides
  • Nuclear Proteins