12 cases of chronic gonorrhoeae prostatitis (CGP) were diagnosed with polymerase chain reaction (PCR). The PCR results were compared with these of culture of prostate fluid (CPF). PCR took 5 hours with a positive rate of 100% and CPF took 3-15 days with a positive rate of 50%. It was shown that PCR is superior to CPF and is a very good method for the diagnosis of CGP.