Nearest neighbor analysis of D1 and D2 subunits in the photosystem II reaction center using a bifunctional cross-linker, hexamethylene diisocyanate

FEBS Lett. 1994 Aug 29;351(1):27-30. doi: 10.1016/0014-5793(94)00803-5.

Abstract

A cross-linked product between the D1 and D2 subunits was generated by treating isolated spinach PS II reaction center with a bifunctional cross-linker, 1,6-hexamethylene diisocyanate. To obtain information on the contact site(s) between D1 and D2 proteins, the cross-linked product was cleaved by CNBr, the resultant fragments were separated using a reverse-phase HPLC, and then the partial sequence of the cross-linked polypeptide fragments were determined. By comparing the results with the deduced amino acid sequence of the D1 and D2 proteins from spinach, it is concluded that the C-terminal domains of the D1 subunit (D308-A334) and that of the D2 subunit (Y297-L353) are in close proximity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cross-Linking Reagents
  • Cyanates / chemistry*
  • Isocyanates
  • Molecular Sequence Data
  • Photosynthetic Reaction Center Complex Proteins / chemistry*
  • Photosystem II Protein Complex

Substances

  • Cross-Linking Reagents
  • Cyanates
  • Isocyanates
  • Photosynthetic Reaction Center Complex Proteins
  • Photosystem II Protein Complex
  • 1,6-hexamethylene diisocyanate