Abstract
A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat sequence, but displays one less consensus site for asparagine N-linked glycosylation and one more consensus site for phosphorylation by protein kinase C. The human SVMT gene maps to chromosome 10q25 using Southern blotting analysis of human/rodent hybrid cell lines and fluorescent in situ hybridization approaches. The cDNA, and a subclone, recognize TaqI polymorphisms that may prove useful to assess this gene's involvement in neuropsychiatric disorders involving monoaminergic brain systems.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Chromosome Mapping
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Chromosomes, Human, Pair 10*
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Consensus Sequence
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DNA / chemistry
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DNA / genetics*
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Deoxyribonucleases, Type II Site-Specific*
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Glycoproteins / chemistry
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Glycoproteins / genetics*
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Glycosylation
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Humans
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In Situ Hybridization
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Membrane Glycoproteins*
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Membrane Transport Proteins*
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Molecular Sequence Data
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Neuropeptides*
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Phosphorylation
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Polymorphism, Restriction Fragment Length*
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Protein Kinase C / metabolism
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Rats
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Sequence Homology, Amino Acid
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Synaptic Vesicles / chemistry*
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Vesicular Biogenic Amine Transport Proteins
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Vesicular Monoamine Transport Proteins
Substances
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Glycoproteins
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Membrane Glycoproteins
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Membrane Transport Proteins
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Neuropeptides
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SLC18A2 protein, human
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Vesicular Biogenic Amine Transport Proteins
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Vesicular Monoamine Transport Proteins
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DNA
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Protein Kinase C
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Deoxyribonucleases, Type II Site-Specific
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TCGA-specific type II deoxyribonucleases