Schistosoma japonicum-infected mice show reduced hepatic fibrosis and eosinophilia and selective inhibition of interleukin-5 secretion by CD4+ cells after treatment with anti-interleukin-2 antibodies

Infect Immun. 1993 Apr;61(4):1288-92. doi: 10.1128/iai.61.4.1288-1292.1993.

Abstract

Schistosoma japonicum-infected mice were injected with antibodies to interleukin-2 (IL-2) and/or IL-2 receptor to clarify the role of IL-2 on the granulomatous reaction around schistosome eggs in the liver. Granulomas were of normal or slightly increased size in animals subjected to IL-2 blockade, but hepatic fibrosis was markedly decreased in treated animals 10 weeks after infection. Anti-IL-2 treatment significantly decreased the in vitro secretion of IL-5 by antigen-stimulated spleen cells, and peripheral eosinophilia and tissue eosinophilia were diminished. Secretion of IL-2, IL-4, and gamma interferon was unaffected. Our results indicate that IL-2 is not an essential determinant of granuloma size in S. japonicum-infected mice but that, as in Schistosoma mansoni infection, the development of hepatic fibrosis is critically dependent on IL-2 levels and granuloma size and hepatic fibrosis are differentially regulated.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies / therapeutic use*
  • CD4-Positive T-Lymphocytes / physiology*
  • Eosinophilia / therapy
  • Female
  • Granuloma / pathology
  • Interleukin-2 / immunology*
  • Interleukin-4 / metabolism
  • Interleukin-5 / metabolism*
  • Liver / pathology
  • Mice
  • Mice, Inbred C3H
  • Schistosomiasis japonica / pathology
  • Schistosomiasis japonica / therapy*
  • T-Lymphocytes, Helper-Inducer / immunology

Substances

  • Antibodies
  • Interleukin-2
  • Interleukin-5
  • Interleukin-4