Abstract
Using both chromosomal in situ hybridization and molecular techniques, we report the genetic localization of the gene coding for the alpha 1 subunit of the skeletal slow Ca2+ current channel/DHP receptor gene (Cchl1a3) on human Chromosome (Chr) 1 (1q31-1q32 region) and on mouse Chr 1 (region (F-G)). On the basis of single-strand conformation polymorphism (SSCP-PCR) analysis in an interspecific backcross, we have determined that the Cchl1a3 = mdg (muscular dysgenesis) locus is very closely linked to the myogenin (Myog) locus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Bone and Bones / metabolism*
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Calcium Channels / genetics*
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Calcium Channels / metabolism
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Calcium Channels, L-Type
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Chromosome Mapping
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Chromosomes, Human, Pair 1*
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Crosses, Genetic
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DNA Primers
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Humans
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In Situ Hybridization
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Muridae
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Muscle Proteins / genetics*
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Polymorphism, Genetic
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Receptors, Cholinergic / genetics*
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Receptors, Cholinergic / metabolism
Substances
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Calcium Channels
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Calcium Channels, L-Type
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DNA Primers
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Muscle Proteins
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Receptors, Cholinergic