Human gene mapping and chromosome sorting by laser technology

N Shimizu; S Minoshima; J Kudoh; K Kawasaki; Y Wang; Y Shimizu; K Sakai

doi:10.2302/kjm.42.212

Human gene mapping and chromosome sorting by laser technology

Keio J Med. 1993 Dec;42(4):212-6. doi: 10.2302/kjm.42.212.

Authors

N Shimizu¹, S Minoshima, J Kudoh, K Kawasaki, Y Wang, Y Shimizu, K Sakai

Affiliation

¹ Department of Molecular Biology, School of Medicine, Keio University, Tokyo, Japan.

PMID: 8126985
DOI: 10.2302/kjm.42.212

Abstract

More than 30 human genes have been mapped by a combination of the fluorescent in situ hybridization (FISH) and spot blot hybridization in this laboratory for the past five years. Furthermore, the Keio strategy has been established for the molecular analysis of the human genome using flow-sorted human chromosomes.

MeSH terms

Base Sequence
Cell Separation / instrumentation
Chromosome Mapping / instrumentation
Chromosome Mapping / methods*
Cosmids
Flow Cytometry / instrumentation
Flow Cytometry / methods*
Gene Library
Genome, Human*
Humans
Immunoblotting / instrumentation
Immunoblotting / methods
In Situ Hybridization, Fluorescence / instrumentation
In Situ Hybridization, Fluorescence / methods
Lasers*
Molecular Sequence Data
Sequence Analysis, DNA / instrumentation