[A universal method of purifying genetically engineered human somatotropin and its mutants using metallochelating agent affinity chromatography]

S A Lopatin; A V Il'ina; A A Shul'ga; O S Grinchenko; V P Varlamov; K G Skriabin; M P Kirpichnikov

[A universal method of purifying genetically engineered human somatotropin and its mutants using metallochelating agent affinity chromatography]

Biokhimiia. 1994 Feb;59(2):226-30.

[Article in Russian]

Authors

S A Lopatin, A V Il'ina, A A Shul'ga, O S Grinchenko, V P Varlamov, K G Skriabin, M P Kirpichnikov

PMID: 8155782

Abstract

A novel scheme for human recombinant somatotropin and its mutant forms isolation which includes ion-exchange and metal chelate affinity chromatography has been developed. A change in the chromatographic behaviour of the isolated proteins depending on the amino acid substitutions has been demonstrated.

Publication types

English Abstract

MeSH terms

Chelating Agents
Chromatography, Affinity / methods
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Growth Hormone / genetics
Growth Hormone / isolation & purification*
Humans
Metals
Mutation*
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification

Substances

Chelating Agents
Metals
Recombinant Proteins
Growth Hormone