Isolation and mapping of microsatellites from a library microdissected from the Werner syndrome region, 8p11.2-p22

Jpn J Hum Genet. 1993 Dec;38(4):391-7. doi: 10.1007/BF01907985.

Abstract

We have constructed a new genetic linkage map of the Werner syndrome (WRN) region, using microsatellites from a library which was developed by a chromosome microdissection and enzymatic amplification method. These microsatellites were used to genotype members of CEPH families using a simplified detection system of polymerase chain reaction (PCR) products. Two-point analysis was used to assign 4 microsatellite markers relative to each marker and other markers reported in the CEPH public data base. We confirmed that these 4 markers are located to the WRN region, 8p11.2-p22. Such microsatellites microdissected from the definite chromosome region may be useful for positional cloning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 8*
  • DNA, Satellite / genetics
  • DNA, Satellite / isolation & purification*
  • Genetic Linkage
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Werner Syndrome / genetics*

Substances

  • DNA, Satellite