Determination of threshold UV-A elicitation dose in photopatch testing

Contact Dermatitis. 1993 Oct;29(4):192-4. doi: 10.1111/j.1600-0536.1993.tb03536.x.

Abstract

Photopatch testing, although widely used in the diagnosis of photosensitivity disorders, is not standardized. We performed this study to determine the threshold ultraviolet light A (UV-A) dose required to elicit photopatch test responses. 4 patients with previously positive tests were reexposed to the offending allergen, using an incremental dosage regime. Isopropyl dibenzoylmethane (Eusolex 8020), mexenone (benzophenone-10) and oxybenzone (benzophenone-3) produced positive responses at 1.0, 1.0 and 0.7 J/cm2, respectively. Responses to phenothiazines were deemed phototoxic. These results demonstrate that high doses of UV-A (e.g., 10-15 J/cm2) are unnecessary, and that 5 J/cm2 should become the current standard.

MeSH terms

  • Adult
  • Allergens / immunology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Patch Tests / methods*
  • Photosensitivity Disorders / diagnosis*
  • Photosensitivity Disorders / etiology
  • Photosensitivity Disorders / immunology
  • Radiation Dosage
  • Reference Standards
  • Skin / immunology
  • Ultraviolet Rays* / adverse effects

Substances

  • Allergens