Transgenic mice and transhybridomas producing chimeric mouse/human anti-human interleukin-2 receptor recombinant antibodies

Gene. 1993 Dec 27;137(1):101-7. doi: 10.1016/0378-1119(93)90257-4.

Abstract

Transgenic mice were developed that secreted chimeric mouse/human anti-human interleukin-2 receptor (IL-2R) antibodies (Ab) into their serum. In addition, hybridomas producing the chimeric Ab in tissue culture were generated from the transgenic mice. The presence of the mouse/human immunoglobulin (Ig) transgene did not appear to affect rearrangement of endogenous murine Ig in the hybridomas. Serum levels of the chimeric Ab correlated with transgene copy number. Although many of the transgenic lineages had serum titers of the chimeric Ab comparable to endogenous mouse IgG, there was no apparent correlation with endogenous mouse IgG levels.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Base Sequence
  • Culture Techniques
  • Humans
  • Hybridomas
  • IgG Deficiency / genetics
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Receptors, Interleukin-2 / immunology*
  • Recombinant Proteins / immunology
  • Spleen / metabolism

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Receptors, Interleukin-2
  • Recombinant Proteins