Phospholipase D: a downstream effector of ARF in granulocytes

Science. 1994 Jan 28;263(5146):523-6. doi: 10.1126/science.8290961.

Abstract

Activation of the phospholipase D (PLD) pathway is a widespread response when cells are activated by agonists that bind receptors on the cell surface. A 16-kD cytosolic component can reconstitute guanosine triphosphate (GTP)-mediated activation of phospholipase D in HL60 cells depleted of their cytosol by permeabilization. This factor was purified and identified as two small GTP-binding proteins, ARF1 and ARF3. Recombinant ARF1 substituted for purified ARF proteins in the reconstitution assay. These results indicate that phospholipase D is a downstream effector of ARF1 and ARF3. The well-established role of ARF in vesicular traffic would suggest that alterations in lipid content by PLD are an important determinant in vesicular dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-Ribosylation Factor 1
  • ADP-Ribosylation Factors
  • Amino Acid Sequence
  • Animals
  • Cattle
  • Cytosol / chemistry
  • Enzyme Activation
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / isolation & purification
  • GTP-Binding Proteins / metabolism*
  • Glycerophospholipids*
  • Granulocytes / metabolism*
  • Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
  • Humans
  • Molecular Sequence Data
  • Phosphatidic Acids / metabolism
  • Phosphatidylcholines / metabolism
  • Phospholipase D / metabolism*
  • Recombinant Proteins / metabolism
  • Tumor Cells, Cultured

Substances

  • Glycerophospholipids
  • Phosphatidic Acids
  • Phosphatidylcholines
  • Recombinant Proteins
  • phosphatidylethanol
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Phospholipase D
  • GTP-Binding Proteins
  • ADP-Ribosylation Factor 1
  • ADP-Ribosylation Factors