Abstract
Three disulfide linkages of recombinant human brain-derived neurotrophic factor (BDNF) were determined by peptide sequence analysis and characterized by mass spectrometry. The three disulfide bonds for BDNF expressed in Chinese hamster ovary cells include Cys-13-Cys-80, Cys-58-Cys-109 and Cys-68-Cys-111, and the disulfide structure was homologous to that of nerve growth factor.
MeSH terms
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Amino Acid Sequence
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Animals
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Brain-Derived Neurotrophic Factor
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CHO Cells
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Cricetinae
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Cystine / chemistry
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Humans
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Molecular Sequence Data
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Nerve Growth Factors / chemistry*
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Nerve Growth Factors / metabolism
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Nerve Tissue Proteins / chemistry*
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Nerve Tissue Proteins / metabolism
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Recombinant Proteins / chemistry
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Sequence Analysis
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Thermolysin / metabolism
Substances
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Brain-Derived Neurotrophic Factor
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Nerve Growth Factors
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Nerve Tissue Proteins
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Recombinant Proteins
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Cystine
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Thermolysin