Effects of cadmium on potassium currents in activated B lymphocytes

Cell Signal. 1993 Jul;5(4):417-24. doi: 10.1016/0898-6568(93)90081-v.

Abstract

We have applied the patch clamp technique in the whole-cell configuration to study whole-cell currents in B lymphocytes under three conditions: (i) resting; (ii) interleukin-4 (IL-4)-treated; and (iii) IL-4 plus cadmium-treated murine B lymphocytes. Through these experiments we have: (i) confirmed our earlier findings and the observation of others that resting B cells express only outward currents; (ii) confirmed the presence of an inwardly rectifying K+ current elicited by treatment with the lymphokine IL-4 that was revealed in our previous study on single channel currents; (iii) demonstrated that both inward and outward rectifying K+ currents in IL-4-treated B cells are dramatically reduced by exposure to 20 microM cadmium; and (iv) determined that the activation curve of the IL-4-induced inward rectifier is shifted to more negative voltages by cadmium. We propose that one of the mechanisms by which cadmium can mediate toxicity in activated B lymphocytes is through the suppression and modulation of potassium currents, effects that may alter the timing of entry into the cell cycle.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology
  • B-Lymphocytes / physiology*
  • Cadmium / pharmacology*
  • In Vitro Techniques
  • Interleukin-4 / pharmacology
  • Lymphocyte Activation
  • Membrane Potentials / drug effects
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Potassium Channels / drug effects
  • Potassium Channels / physiology*
  • Spleen / physiology

Substances

  • Potassium Channels
  • Cadmium
  • Interleukin-4