Characterization of clonality of Epstein-Barr virus-induced human B lymphoproliferative disease in mice with severe combined immunodeficiency

Am J Pathol. 1993 Jan;142(1):139-47.

Abstract

To improve the diagnostic accuracy and understanding of the pathogenesis of lymphoproliferative diseases (LPDs) occurring in immunosuppressed transplant recipients (post-transplantation LPD), clonality of Epstein-Barr virus-induced human LPDs in mice with severe combined immunodeficiency was examined by analyzing: 1) human immunoglobulin genes and their products, 2) the clonality of Epstein-Barr virus DNA, and 3) genetic alteration of c-myc or bcl-2 genes. A spectrum of clonality was found in the LPDs comparable with that reported for post-transplantation LPDs, although rearrangements of c-myc or bcl-2 genes were not detected. It is confirmed that this system is useful in terms of clonality for understanding the early phases in the pathogenesis of post-transplantation LPD or LPD in immune deficient patients.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / microbiology
  • Cell Transformation, Viral
  • Genes, Immunoglobulin
  • Genome, Viral
  • Herpesvirus 4, Human* / genetics
  • Herpesvirus 4, Human* / immunology
  • Humans
  • Immunoelectrophoresis, Two-Dimensional
  • Immunophenotyping
  • Lymphoproliferative Disorders / etiology
  • Lymphoproliferative Disorders / genetics
  • Lymphoproliferative Disorders / immunology
  • Mice
  • Mice, SCID
  • Severe Combined Immunodeficiency / etiology
  • Severe Combined Immunodeficiency / genetics*
  • Severe Combined Immunodeficiency / immunology*