Structure of a major immunogenic site on foot-and-mouth disease virus

Nature. 1993 Apr 8;362(6420):566-8. doi: 10.1038/362566a0.

Abstract

Attachment of foot-and-mouth disease virus (FMDV) to its cellular receptor involves a long and highly antigenic loop containing the conserved sequence, Arg-Gly-Asp, a motif known to be a recognition element in many integrin-dependent cell adhesion processes. In our original crystal structure of FMDV the Arg-Gly-Asp-containing loop ('the loop'), located between beta-strands G and H of capsid protein VP1, was disordered and hence essentially invisible. We previously surmised that its disorder is enhanced by a disulphide bond linking the base of the loop (Cys 134) to Cys 130 of VP2 (ref. 8). We report here the crystal structure of the virus in which this disulphide is reduced. Reduced virus retains infectivity and serological experiments suggest that some of the loop's internal structure is conserved. But here its structure has become sufficiently ordered to allow us to describe an unambiguous conformation, which we relate to some key biological properties of the virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aphthovirus / chemistry*
  • Capsid / chemistry
  • Capsid Proteins
  • Molecular Conformation
  • Molecular Structure
  • Oligopeptides

Substances

  • Capsid Proteins
  • Oligopeptides
  • VP1 protein, Foot-and-mouth disease virus
  • arginyl-glycyl-aspartic acid