Abstract
A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a starting point for the design of an HCV single all-encompassing antigen for a blood screening assay.
MeSH terms
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Antigens, Viral / chemistry
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Antigens, Viral / genetics*
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Antigens, Viral / immunology
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Cloning, Molecular
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Hepacivirus / chemistry
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Hepacivirus / immunology*
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Hepatitis Antibodies / immunology
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Recombinant Fusion Proteins / immunology
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Viral Core Proteins / chemistry
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Viral Core Proteins / genetics
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Viral Core Proteins / immunology*
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Viral Nonstructural Proteins / chemistry
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Viral Nonstructural Proteins / immunology*
Substances
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Antigens, Viral
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Hepatitis Antibodies
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Recombinant Fusion Proteins
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Viral Core Proteins
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Viral Nonstructural Proteins