Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons

Microb Pathog. 1993 Jul;15(1):77-82. doi: 10.1006/mpat.1993.1058.

Abstract

The polymerase chain reaction (PCR) was used to amplify approximately 1.5 kb segments of DNA containing complete Shiga-like toxin type II operons from Escherichia coli serotypes OX3:H21 and O111:H-. These fragments were cloned and DNA sequence analysis identified further variations compared with published SLT-II sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • Escherichia coli / genetics*
  • Genetic Variation*
  • Genome, Bacterial
  • Molecular Sequence Data
  • Operon / genetics*
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Shiga Toxin 2

Substances

  • Bacterial Toxins
  • DNA Primers
  • Shiga Toxin 2

Associated data

  • GENBANK/L11078
  • GENBANK/L11079
  • GENBANK/M59432
  • GENBANK/X65949