The internal lateral (IL) subnucleus of the parabrachial nucleus (PB), which is one of the seven lateral subnuclei of the PB, receives information from the spinal cord. The IL subnucleus perhaps relays nociceptive signals to the intralaminar nuclei of the thalamus, apparently being implicated in the motivational-affective component of pain reaction. However, cells of origin of spinal fibers to the IL subnucleus have not been investigated sufficiently. We intended to clarify these cells by injection of fast blue or wheat germ agglutinin-conjugated horseradish peroxidase into the IL subnucleus and/or other lateral subnuclei in the rat. When the tracer was injected into the IL subnucleus, many cells were labeled bilaterally in laminae I, V, and VII, and in the dorsolateral and dorsomedial parts of the lateral funiculus throughout the entire length of the spinal cord. A small number of labeled cells appeared ipsilaterally in laminae II-IV and VI in the upper cervical segments and contralaterally in laminae VIII and X throughout the spinal cord. Labeled cells in lamina I were more numerous ipsilaterally than contralaterally in the first two cervical segments but were more numerous contralaterally than ipsilaterally in the remaining spinal segments. Labeled cells were seen with a contralateral predominance in lamina VII, but with an ipsilateral predominance in lamina V and in the dorsolateral and dorsomedial parts of the lateral funiculus. With tracer injected into the lateral subnuclei of the PB, excluding the IL subnucleus, labeled cells were found primarily in lamina I throughout the entire length of the spinal cord. These results show that cells giving rise to spinoparabrachial fibers were more numerous and more widely distributed than previously reported.