A re-examination of the molecular basis of cell movement

Immunol Cell Biol. 1993 Apr:71 ( Pt 2):131-9. doi: 10.1038/icb.1993.14.

Abstract

A model for cell movement is presented. It is suggested that cells do not migrate on collagen using their VLA (very late antigen) integrins that bind this extracellular matrix protein. Rather, the cells utilize alpha v integrins to bind endogenously produced fibronectin, which binds to the underlying collagen. It is envisaged that cells proceed by a process of engagement and disengagement of alpha v integrins to the extracellular matrix, somewhat analogous to the motion of a monkey climbing a tree. Secretion of isoforms of the adhesion modulator, thrombospondin, regulates disengagement of the integrin from its ligand in migrating cells. The integrin disengagement signal is mediated by thrombospondin cross-linking the alpha v integrin to an integrin accessory molecule and thus activating protein kinases. The cross-linked receptor complex undergoes recycling back along actin stress fibres, guided by the integrin beta-subunit. After endocytosis and protein sorting the alpha v integrin is transported back to the leading edge off migrating cells in vesicles guided by the tubulin-binding capabilities of an integrin accessory molecule. Direct attachment to collagen required for processes, such as matrix contraction, is mediated by VLA integrins which displace alpha v integrins from points of attachment during integrin recycling, possibly through an alpha v beta 1 intermediary receptor.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Adhesion / physiology
  • Cell Movement / physiology*
  • Collagen / physiology
  • Extracellular Matrix / physiology
  • Humans
  • Integrins / physiology
  • Microtubules / physiology
  • Models, Biological

Substances

  • Integrins
  • Collagen