Hexose uptake of the transformed lymphocyte cell line IM9 was analysed with respect to kinetics, responsiveness to insulin and insulin-like growth factor I and to cell cycle dependency. We found a Km of 0.76 mM and a Vmax of 14.9 nMol/min/mg protein for 2-deoxy-D-[3H]glucose uptake. This Km is in accordance with the values described for the erythrocyte type and the brain type glucose transporter. Hexose uptake of IM9 lymphocytes was not altered by physiological concentrations of insulin or IGF I. We investigated the 2-deoxy-D-[3H]glucose transport in cells, enriched in the different cell cycle phases by counterflow elutriation. The results were calculated for hypothetical pure fractions. Hexose uptake per cell is more than doubled during the S-phase and returns in G2-cells to the basal value of G1-phase.