Assay and expression of mitogen-activated protein kinase, MAP kinase kinase, and Raf

Methods Enzymol. 1995:255:279-90. doi: 10.1016/s0076-6879(95)55031-3.

Authors

D R Alessi¹, P Cohen, A Ashworth, S Cowley, S J Leevers, C J Marshall

Affiliation

¹ Department of Biochemistry, University of Dundee, Scotland.

PMID: 8524112
DOI: 10.1016/s0076-6879(95)55031-3

No abstract available

MeSH terms

Amino Acid Sequence
Animals
Calcium-Calmodulin-Dependent Protein Kinases / analysis*
Calcium-Calmodulin-Dependent Protein Kinases / genetics
Calcium-Calmodulin-Dependent Protein Kinases / isolation & purification
Escherichia coli / genetics
Gene Expression
Mitogen-Activated Protein Kinase Kinases
Molecular Sequence Data
PC12 Cells
Peptides / genetics
Phosphorylation
Protein Kinases / analysis*
Protein Kinases / genetics
Protein Kinases / isolation & purification
Protein Serine-Threonine Kinases / analysis*
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / isolation & purification
Proto-Oncogene Proteins / analysis*
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / isolation & purification
Proto-Oncogene Proteins c-raf
Rabbits
Rats
Recombinant Fusion Proteins / analysis
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / isolation & purification

Substances

Peptides
Proto-Oncogene Proteins
Recombinant Fusion Proteins
Protein Kinases
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins c-raf
Calcium-Calmodulin-Dependent Protein Kinases
Mitogen-Activated Protein Kinase Kinases