Abstract
The impact of Listeria monocytogenes listeriolysin O (LLO) secretion on phosphoinositide metabolism and mediator (platelet-activating factor and prostaglandin I2) generation was investigated in human umbilical vein endothelial cells. Wild-type L. monocytogenes, purified LLO, and an L. innocua strain engineered to secrete LLO all elicited a strong response, whereas mutant strains defective in LLO production were ineffective. Thus, human umbilical vein endothelial cell stimulation by listeriae is linked to production of LLO.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Toxins*
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Cells, Cultured
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Endothelium, Vascular / drug effects*
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Endothelium, Vascular / metabolism
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Epoprostenol / biosynthesis*
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Heat-Shock Proteins / pharmacology*
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Hemolysin Proteins / pharmacology*
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Humans
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L-Lactate Dehydrogenase / metabolism
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Phosphatidylinositols / biosynthesis*
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Platelet Activating Factor / biosynthesis*
Substances
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Bacterial Toxins
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Heat-Shock Proteins
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Hemolysin Proteins
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Phosphatidylinositols
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Platelet Activating Factor
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Epoprostenol
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L-Lactate Dehydrogenase
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hlyA protein, Listeria monocytogenes