The v-erbA oncogene selectively inhibits iodide uptake in rat thyroid cells

Oncogene. 1996 May 2;12(9):1879-88.

Abstract

v-erbA is the oncogenic form of the c-erbA proto-oncogene, which encodes the receptor for thyroid hormones. The expression of the v-erbA oncogene in thyroid differentiated cells, PC Cl 3, inhibits iodide uptake and thyrotropin-dependent growth, whereas it has no effect on the expression of the other thyroid specific markers, i.e. thyroglobulin, thyroperoxidase and thyrotropin receptor. The activity of transcription factor AP-1, evaluated by a specific DNA binding assay and by transcription of AP-induced promoter (TRE) is enhanced in PC v-erbA cells. v-erbA mutants in the DNA binding domain do not affect the iodide uptake of thyroid cells nor AP-1 activity. We suggest that this transcriptional activation mediates the selective effects of v-erbA on the expression of thyroid specific markers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Differentiation / genetics
  • Cell Division / genetics
  • Cell Line, Transformed
  • Clone Cells
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Genes, erbA*
  • Iodides / metabolism*
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Rats
  • Rats, Inbred F344
  • Thyroid Gland / cytology
  • Thyroid Gland / metabolism*
  • Transcription Factor AP-1 / metabolism
  • Transfection

Substances

  • DNA-Binding Proteins
  • Iodides
  • Transcription Factor AP-1