Abstract
B and T lymphocytes undergoing apoptosis in response to anti-immunoglobulin M antibodies and dexamethasone, respectively, were found to have increased amounts of messenger RNA for the inositol 1,4,5-trisphosphate receptor (IP3R) and increased amounts of IP3R protein. Immunohistochemical analysis revealed that the augmented receptor population was localized to the plasma membrane. Type 3 IP3R (IP3R3) was selectively increased during apoptosis, with no enhancement of type 1 IP3R (IP3R1). Expression of IP3R3 antisense constructs in S49 T cells blocked dexamethasone-induced apoptosis, whereas IP3R3 sense, IP3R1 sense, or IP3R1 antisense control constructs did not block cell death. Thus, the increases in IP3R3 may be causally related to apoptosis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Apoptosis*
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B-Lymphocytes / cytology*
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B-Lymphocytes / metabolism
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Base Sequence
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Calcium / metabolism
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Calcium Channels / genetics
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Calcium Channels / immunology
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Calcium Channels / metabolism*
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Cell Line
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Cell Membrane / metabolism
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Cells, Cultured
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DNA, Antisense
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Dexamethasone / pharmacology
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Immunoblotting
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Inositol 1,4,5-Trisphosphate / metabolism*
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Inositol 1,4,5-Trisphosphate Receptors
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Mice
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Molecular Sequence Data
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / immunology
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Receptors, Cytoplasmic and Nuclear / metabolism*
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T-Lymphocytes / cytology*
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T-Lymphocytes / metabolism
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Transfection
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Tumor Cells, Cultured
Substances
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Calcium Channels
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DNA, Antisense
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Inositol 1,4,5-Trisphosphate Receptors
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Receptors, Cytoplasmic and Nuclear
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Dexamethasone
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Inositol 1,4,5-Trisphosphate
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Calcium