Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content

Genome Res. 1996 Jul;6(7):633-8. doi: 10.1101/gr.6.7.633.

Abstract

A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.

MeSH terms

  • Base Composition*
  • Betaine
  • Carrier Proteins / genetics
  • DNA / chemistry
  • DNA / genetics*
  • DNA-Directed DNA Polymerase
  • Dimethyl Sulfoxide
  • Female
  • Fragile X Syndrome / genetics
  • Humans
  • Major Histocompatibility Complex / genetics
  • Male
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Organic Cation Transporter 1
  • Polymerase Chain Reaction / methods*
  • Receptors, Transferrin / genetics
  • Templates, Genetic
  • Trinucleotide Repeats

Substances

  • Carrier Proteins
  • Membrane Proteins
  • Organic Cation Transporter 1
  • Receptors, Transferrin
  • Betaine
  • DNA
  • Pfu DNA polymerase
  • DNA-Directed DNA Polymerase
  • Dimethyl Sulfoxide

Associated data

  • GENBANK/L20433
  • GENBANK/X01060
  • GENBANK/X13403