Intraosseous secondary calcium salt crystal deposition: an artefact of acid decalcification

Histopathology. 1995 Aug;27(2):181-5. doi: 10.1111/j.1365-2559.1995.tb00027.x.

Abstract

We previously observed, in decalcificated bone specimens, intraosseous crystal deposits with morphological and analytical similarity to calcium pyrophosphate dihydrate. We have now been able, by a combination of more detailed morphological studies of these and similar cases, and by infrared spectroscopy in three cases, to show that this is, in fact deposition of the secondary calcium salts brushite and monetite, occurring as an artefact during formic acid decalcification. Our earlier postulate of bone as an additional primary crystallization site for calcium pyrophosphate dihydrate is effectively disproved. This artefact deserves wider recognition.

MeSH terms

  • Artifacts
  • Bone and Bones / metabolism
  • Bone and Bones / pathology
  • Calcium Phosphates / analysis*
  • Calcium Phosphates / metabolism
  • Chondrocalcinosis / metabolism*
  • Chondrocalcinosis / pathology
  • Crystallization
  • Humans
  • Spectrum Analysis

Substances

  • Calcium Phosphates
  • alpha-tricalcium phosphate
  • tetracalcium phosphate
  • calcium phosphate, monobasic, anhydrous
  • calcium phosphate
  • calcium phosphate, dibasic, anhydrous
  • calcium phosphate, dibasic, dihydrate