The amino acid substitutions Ser288Gly and Val320Ala convert the cortisol producing enzyme, CYP11B1, into an aldosterone producing enzyme

K M Curnow; P Mulatero; N Emeric-Blanchouin; B Aupetit-Faisant; P Corvol; L Pascoe

doi:10.1038/nsb0197-32

The amino acid substitutions Ser288Gly and Val320Ala convert the cortisol producing enzyme, CYP11B1, into an aldosterone producing enzyme

Nat Struct Biol. 1997 Jan;4(1):32-5. doi: 10.1038/nsb0197-32.

Authors

K M Curnow, P Mulatero, N Emeric-Blanchouin, B Aupetit-Faisant, P Corvol, L Pascoe

PMID: 8989319
DOI: 10.1038/nsb0197-32

Abstract

Transfection studies with cDNAs encoding hybrids between the highly similar cytochrome P450 enzymes, CYP11B1 (steroid 11 beta-hydroxylase) and CYP11B2 (aldosterone synthase) have identified which amino acids determine the different activities of the enzymes.

Publication types

Letter

MeSH terms

Aldosterone / biosynthesis*
Amino Acid Sequence
Amino Acids / genetics*
DNA, Complementary
Humans
Hydrocortisone / biosynthesis*
Mitochondria / enzymology
Molecular Sequence Data
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Steroid 11-beta-Hydroxylase / chemistry
Steroid 11-beta-Hydroxylase / genetics
Steroid 11-beta-Hydroxylase / metabolism*
Transfection

Substances

Amino Acids
DNA, Complementary
Aldosterone
Steroid 11-beta-Hydroxylase
Hydrocortisone