Cloning and sequence analysis of putative histidine protein kinases isolated from Lactococcus lactis MG1363

Appl Environ Microbiol. 1997 Jun;63(6):2454-9. doi: 10.1128/aem.63.6.2454-2459.1997.

Abstract

Eight recombinant plasmids harboring chromosomal fragments of Lactococcus lactis MG1363 were shown to phenotypically suppress a histidine protein kinase (HPK) deficiency in either of two different E. coli strains. Sequence analysis of the plasmid inserts revealed five different complete or partial open reading frames (ORFs) specifying proteins with high similarity to HPKs. One of the plasmids also harbored an additional ORF, unrelated to HPKs, with suppressing activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Genes, Bacterial
  • Genetic Complementation Test
  • Histidine Kinase
  • Lactococcus lactis / enzymology*
  • Lactococcus lactis / genetics*
  • Molecular Sequence Data
  • Mutation
  • Open Reading Frames
  • Phenotype
  • Plasmids / genetics
  • Protein Kinases / genetics*
  • Recombination, Genetic
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Suppression, Genetic

Substances

  • DNA, Bacterial
  • Protein Kinases
  • Histidine Kinase

Associated data

  • GENBANK/U81166
  • GENBANK/U81485
  • GENBANK/U81486
  • GENBANK/U81487
  • GENBANK/U81488
  • GENBANK/U81489