Occupational transmission of hepatitis B virus (HBV), hepatitis C virus, and HIV has been documented. The risk for occupationally transmitted infection varies for these three viruses. Despite effective pre- and postexposure prophylaxis for HBV and recent recommendations for postexposure chemoprophylaxis after an HIV exposure, the best approach to prevent occupational bloodborne infection is the prevention of blood exposures. Epidemiologic data of percutaneous injuries and other blood contacts have provided the basis for prevention strategies. These strategies include the development of improved engineering controls, work practices, and personal protective equipment.