Abstract
Some AMPA receptors are permeable to Ca2+. It has been suggested that cultured rat cerebellar granule cells express Ca(2+)-permeable AMPA receptors, but their distribution at a single cell level is unknown. We report that AMPA (in the presence of cyclothiazide) induced Ca2+ entry (measured by Mn2+ quench of fura-2 fluorescence) and intracellular Ca2+ increases in cerebellar granule cells in the absence of extracellular Na+, supporting the presence of Ca(2+)-permeable AMPA receptors. Analysis of intracellular Ca2+ signals in single cells demonstrated a heterogeneous distribution of Ca(2+)-permeable AMPA receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzothiadiazines / pharmacology
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Calcium / metabolism*
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Cells, Cultured
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Cerebellum / cytology
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Cerebellum / metabolism*
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Culture Media
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Culture Media, Serum-Free
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Diuretics
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Dizocilpine Maleate / pharmacology
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Excitatory Amino Acid Agonists / pharmacology
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Excitatory Amino Acid Antagonists / pharmacology
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Fluorescent Dyes
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Fura-2
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Manganese / metabolism
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Quinoxalines / pharmacology
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Rats
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Receptors, AMPA / metabolism*
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Sodium Chloride Symporter Inhibitors / pharmacology
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology
Substances
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Benzothiadiazines
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Culture Media
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Culture Media, Serum-Free
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Diuretics
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Excitatory Amino Acid Agonists
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Excitatory Amino Acid Antagonists
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Fluorescent Dyes
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Quinoxalines
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Receptors, AMPA
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Sodium Chloride Symporter Inhibitors
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Manganese
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FG 9041
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Dizocilpine Maleate
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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
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cyclothiazide
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Calcium
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Fura-2