Distribution of Ca(2+)-permeable AMPA receptors among cultured rat cerebellar granule cells

Neuroreport. 1997 May 27;8(8):1877-82. doi: 10.1097/00001756-199705260-00017.

Abstract

Some AMPA receptors are permeable to Ca2+. It has been suggested that cultured rat cerebellar granule cells express Ca(2+)-permeable AMPA receptors, but their distribution at a single cell level is unknown. We report that AMPA (in the presence of cyclothiazide) induced Ca2+ entry (measured by Mn2+ quench of fura-2 fluorescence) and intracellular Ca2+ increases in cerebellar granule cells in the absence of extracellular Na+, supporting the presence of Ca(2+)-permeable AMPA receptors. Analysis of intracellular Ca2+ signals in single cells demonstrated a heterogeneous distribution of Ca(2+)-permeable AMPA receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzothiadiazines / pharmacology
  • Calcium / metabolism*
  • Cells, Cultured
  • Cerebellum / cytology
  • Cerebellum / metabolism*
  • Culture Media
  • Culture Media, Serum-Free
  • Diuretics
  • Dizocilpine Maleate / pharmacology
  • Excitatory Amino Acid Agonists / pharmacology
  • Excitatory Amino Acid Antagonists / pharmacology
  • Fluorescent Dyes
  • Fura-2
  • Manganese / metabolism
  • Quinoxalines / pharmacology
  • Rats
  • Receptors, AMPA / metabolism*
  • Sodium Chloride Symporter Inhibitors / pharmacology
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology

Substances

  • Benzothiadiazines
  • Culture Media
  • Culture Media, Serum-Free
  • Diuretics
  • Excitatory Amino Acid Agonists
  • Excitatory Amino Acid Antagonists
  • Fluorescent Dyes
  • Quinoxalines
  • Receptors, AMPA
  • Sodium Chloride Symporter Inhibitors
  • Manganese
  • FG 9041
  • Dizocilpine Maleate
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
  • cyclothiazide
  • Calcium
  • Fura-2