A randomized animal study evaluating the efficacies of locally delivered heparin and urokinase for reducing in-stent restenosis

Coron Artery Dis. 1997 May;8(5):293-8. doi: 10.1097/00019501-199705000-00006.

Abstract

Background: In-stent restenosis is primarily due to neointimal hyperplasia. Results from recent nonrandomized studies suggest that local delivery of heparin or urokinase to the site of angioplasty or stenting results in a lower rate of restenosis.

Objective: To determine whether local delivery of heparin or urokinase reduces in-stent restenosis.

Methods and results: Thirty-three pigs were assigned randomly to one of three groups: controls (n = 9) administered local saline infusion, the heparin group (n = 15) administered local heparin (6000 u/10 min), and the urokinase group (n = 9) administered local urokinase (250000 u/10 min), via a local delivery catheter (Dispatch) at the site of subsequent stent implantation. Prior to local delivery, all of the animals were subjected to balloon injury (balloon:artery diameter ratio approximately or = 1.3) to facilitate intramural drug impregnation. After local therapy, one Palmaz-Schatz stent (mean stent: artery diameter ratio approximately or = 1.25) was implanted within the left anterior descending coronary artery. The degree of neointimal hyperplasia was evaluated 4 weeks later by angiography (as the maximal percentage diameter stenosis) and histology (as the maximal neointimal area stenosis). We found no difference in percentage diameter stenosis (46 +/- 18% control, 42 +/- 27% heparin group, and 37 +/- 20% urokinase group, P = 0.7) and corrected neointimal area (1.06 +/- 0.42 mm2 control, 0.94 +/- 0.29 mm2 heparin, and 0.88 +/- 0.26 mm2 urokinase group, P = 0.7) among groups at follow-up. The activated clotting time rose slightly for heparin-treated animals, suggesting that systemic delivery had occurred, whereas fibrinogen levels did not change in urokinase-treated animals.

Conclusions: Local deliveries of heparin and urokinase via the Dispatch catheter, at the chosen dosages, do not reduce in-stent neointimal hyperplasia in this porcine model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Constriction, Pathologic
  • Coronary Disease / prevention & control*
  • Coronary Vessels / pathology*
  • Evaluation Studies as Topic
  • Fibrinolytic Agents / administration & dosage*
  • Heparin / administration & dosage*
  • Hyperplasia / prevention & control
  • Infusions, Intravenous
  • Plasminogen Activators / administration & dosage*
  • Random Allocation
  • Recurrence
  • Stents*
  • Swine
  • Tunica Intima / pathology
  • Urokinase-Type Plasminogen Activator / administration & dosage*

Substances

  • Fibrinolytic Agents
  • Heparin
  • Plasminogen Activators
  • Urokinase-Type Plasminogen Activator